DNA sequencing

Describe the limitations of traditional methods of Identifying bacterium. Traditional methods acknowledge judging by phenotypes characteristics. This could be a problem if two bacteria looked similar because they could easily be confused. Summarize the goal of each of the cardinal parts of the lab. Sample Prep First we need to extract the desoxyribonucleic acid from the bacterial wall PC Amplification Create millions of copies of the initial desoxyribonucleic acid PC purification Filter out deoxyribonucleic acid from primers and enzymes Sequencing Prep drop dead more PC to make copies of desoxyribonucleic acid at various lengths DNA SequencingSequence machine emissions gel electrophoresis to Identify nucleotides and determine sequence. Sequence depth psychology Computer determines full DNA sequence which feces be run and comp bed. Explain how fluorescent markers help determine a nucleotide sequence. each nucleotide is assigned a certain color. Conclusion 1 How can scientists i dentify specific bacteria when they are amplifying and studying the same locality of DNA In each species? They can identify it because there are unique sequences for each species. If you take a sample and find the DNA of it you can then compare it to the Donas of known bacteria to discover what bacteria the sample is from